G-四联体

G-四联体（英语：G-quadruplex、G-tetrads或G₄-DNA）是一种由富含鸟嘌呤的核酸序列所构成的四股型态。含有经由胡斯坦(Hoogsteen)氢键维持稳定，并由鸟嘌呤以正方形方式排列而成的结构。在方形中央有一个单价阳离子（通常是钾）^[1]。这种结构可见于DNA、RNA、LNA（锁核酸）与PNA（肽核酸）；根据形成四联体的各股的方向，可能有分子内（intramolecular）、双分子（bimolecular）或四分子（tetramolecular）等不同类型^[2]。

在四联体的结构中，我们可以根据四股的方向，将结构分为平行和反平行（英语：Antiparallel (biochemistry)）。

Structure of a G-quadruplex. Left: a G-tetrad. Right: an intramolecular G-quadruplex

3D Structure of the intramolecular human telomeric G-quadruplex in potassium solution (PDB ID 2HY9). The backbone is represented by a tube. The center of this structure contains three layers of G-tetrads. The hydrogen bonds in these layers are represented by blue dashed lines.

注释

^ Campbell, Nancy H.; Neidle, Stephen. Chapter 4. G-Quadruplexes and Metal Ions. Astrid Sigel, Helmut Sigel and Roland K. O. Sigel (编). Interplay between Metal Ions and Nucleic Acids. Metal Ions in Life Sciences 10. Springer. 2012: 119–1134. doi:10.1007/978-94-007-2172-2_4.
^ Bochman, Matthew L.; Paeschke, Katrin; Zakian, Virginia A. DNA secondary structures: stability and function of G-quadruplex structures. Nature Reviews Genetics 13. Nature Publishing Group. 2012: 770–780. doi:10.1038/nrg3296.

参考文献

Jiangtao Ren, Jiahai Wang* , Lei Han, Erkang Wang*, Jin Wang*. Kinetically grafting G-qaudruplex onto DNA nanostructure as structure and function encoding via DNA machine. Chem. Commun. 2011, 47: 10563–10565 [2015-06-19]. （原始内容存档于2015-04-20）.
Johnson JE, Smith JS, Kozak ML, Johnson FB. In vivo veritas: using yeast to probe the biological functions of G-quadruplexes. Biochimie. 2008, 90 (8): 1250–1263 [2015-06-19]. PMC 2585026  . PMID 18331848. doi:10.1016/j.biochi.2008.02.013. （原始内容存档于2019-02-07）.
Huppert JL and Balasubramanian S. Prevalence of quadruplexes in the human genome. NAR. 2005, 33 (9): 2908–2916 [2007-06-20]. PMC 1140081  . PMID 15914667. doi:10.1093/nar/gki609. （原始内容存档于2007-05-06）.
Todd AK, Johnston M, Neidle S. Highly prevalent putative quadruplex sequence motifs in human DNA. NAR. 2005, 33 (9): 2901–2907 [2007-06-20]. PMC 1140077  . PMID 15914666. doi:10.1093/nar/gki553. （原始内容存档于2007-05-06）.
Burge S, Parkinson GN, Hazel P, Todd AK, Neidle S. Quadruplex DNA: sequence, topology and structure. NAR. 2006, 34 (19): 5402–5415 [2007-06-20]. PMC 1636468  . PMID 17012276. doi:10.1093/nar/gkl655. （原始内容存档于2008-10-07）.
Siddiqui-Jain A, Grand CL, Bearss DJ, Hurley LH. Direct evidence for a G-quadruplex in a promoter region and its targeting with a small molecule to repress c-MYC transcription. PNAS. 2002, 99 (18): 11593–8 [2007-06-20]. PMC 129314  . PMID 12195017. doi:10.1073/pnas.182256799. （原始内容存档于2007-10-01）.
Rawal P, Kummarasetti VB, Ravindran J, Kumar N, Halder K, Sharma R, Mukerji M, Das SK, Chowdhury S. Genome-wide prediction of G4 DNA as regulatory motifs: Role in Escherichia coli global regulation. Genome Res. 2006, 16 (5): 644–55 [2007-06-20]. PMC 1457047  . PMID 16651665. doi:10.1101/gr.4508806. （原始内容存档于2007-09-12）.
Xu Hou, Wei Guo, Fan Xia, Fu-Qiang Nie, Hua Dong, Ye Tian, Liping Wen, Lin Wang, Liuxuan Cao, Yang Yang, Jianming Xue, Yanlin Song, Yugang Wang, Dongsheng Liu, and Lei Jiang. A biomimetic potassium responsive nanochannel: G-quadruplex DNA conformational switching in a synthetic nanopore. J. Am. Chem. Soc. 2009, 131 (22): 7800–7805 [2015-06-19]. PMID 19435350. doi:10.1021/ja901574c. （原始内容存档于2022-03-15）.
Neidle & Balasubramanian (编). Quadruplex Nucleic Acids. 2006 [2007-06-20]. ISBN 0-85404-374-8. （原始内容存档于2007-09-30）.

外部链接

Nanopore and Aptamer Biosensor group（页面存档备份，存于互联网档案馆）{NAB group}

四联体网站

COST MP0802 Quadruplex Network – the European network of G-quadruplex community: From first principles to applications
Quadruplex.org – a website to serve the quadruplex community
G-Quadruplex World（页面存档备份，存于互联网档案馆） – a website to discuss publications and other information of interest to those working in the field of G-quadruplexes
Quadbase – downloadable data on predicted G-quadruplexes
Greglist – a database listing potential G-quadruplex regulated genes
Database on Quadruplex information: QuadBase from IGIB
GRSDB（页面存档备份，存于互联网档案馆）- a database of G-quadruplexes near RNA processing sites.
GRS_UTRdb- a database of G-quadruplexes in the UTRs.
G-quadruplex Resource Site（页面存档备份，存于互联网档案馆）
non-B Motif Search Tool at non-B DB- a web server to predict G-quadruplex forming motifs and other non-B DNA forming motifs from users' DNA sequences.

预测G-四联体的工具

Quadparser: Downloadable program for finding putative quadruplex-forming sequences（页面存档备份，存于互联网档案馆） from Balasubramanian's group.
QGRS Mapper: a web-based application for predicting G-quadruplexes in nucleotide sequences and NCBI genes（页面存档备份，存于互联网档案馆） from Bagga's group.
Quadfinder: Tool for Prediction and Analysis of G Quadruplex Motifs in DNA/RNA Sequences from Maiti's group, IGIB, Delhi, India^{[永久失效链接]}

[1] Campbell, Nancy H.; Neidle, Stephen. Chapter 4. G-Quadruplexes and Metal Ions. Astrid Sigel, Helmut Sigel and Roland K. O. Sigel (编). Interplay between Metal Ions and Nucleic Acids. Metal Ions in Life Sciences 10. Springer. 2012: 119–1134. doi:10.1007/978-94-007-2172-2_4.

[2] Bochman, Matthew L.; Paeschke, Katrin; Zakian, Virginia A. DNA secondary structures: stability and function of G-quadruplex structures. Nature Reviews Genetics 13. Nature Publishing Group. 2012: 770–780. doi:10.1038/nrg3296.

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