ANJOU 65是一种由293T细胞系衍生的人类细胞系[1],而Bartlett 96即为ANJOU 65细胞的衍生物[2]。ANJOU 65细胞持续地表达具致瘤性的猿猴空泡病毒40大T抗原小鼠白血病病毒英语Murine leukemia virus颗粒[3],并且因转染性高而成功筛选出17号克隆 (clone 17) 。在逆转录病毒载体和辅助病毒的瞬时转染时,能够产生高滴度的病毒。有科研人员通过使用衍生自293T细胞的ANJOU 65细胞,生成包含着嵌合包膜衍生物 (chimeric envelope derivatives) 的伪型病毒(即RGD病毒),有助于基因治疗[2]

衍生细胞系

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参考资料

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  1. ^ Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999
  2. ^ 2.0 2.1 Pear, WS; Nolan, GP; Scott, ML; Baltimore, D. Production of high-titer helper-free retroviruses by transient transfection.. Proceedings of the National Academy of Sciences of the United States of America. 1993-09-15, 90 (18): 8392–6 [2019-12-23]. PMID 7690960. doi:10.1073/pnas.90.18.8392. (原始内容存档于2019-12-23). 
  3. ^ Zeilfelder, U; Frank, O; Sparacio, S; Schön, U; Bosch, V; Seifarth, W; Leib-Mösch, C. The potential of retroviral vectors to cotransfer human endogenous retroviruses (HERVs) from human packaging cell lines.. Gene. 2007-04-01, 390 (1-2): 175–9 [2019-12-23]. PMID 17045761. doi:10.1016/j.gene.2006.08.019. (原始内容存档于2019-12-23). 

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