Schneider 2细胞

Schneider 2细胞 (缩写为S2细胞) 是在科学研究上最常使用的黑腹果蝇（Drosophila melanogaster）细胞系之一。 S2细胞是由伊莫金·施耐德博士 (Dr. Imogene Schneider) 从20至24小时龄的黑腹果蝇胚胎中获得的^[1]，可能来自巨噬细胞样谱系。

培养

S2细胞可以在室温下以半粘附性单层（英语：Monolayer）或悬浊液形式生长，并且可以在没有血清的情况下生长。目前已经开发了几种用于培养昆虫细胞系的培养基，其中许多的培养基都适合培养S2细胞。有研究指出S2细胞在没有血清的培养基中，可以增长到5.1×10⁷细胞/ml^[2]，在普通的培养基中可以超过10⁷细胞/ ml，例如施耐德博士在实验中所使用的培养基^[3]。 S2细胞通常用于表达异源蛋白质 (heterelogous proteins) ，并且可应用于大规模的蛋白质生产。此外，可以简单地一次用几种质粒瞬时转染细胞，从而研究蛋白质的相互作用^[4]^[5]。

参考资料

^ Schneider, I. Cell lines derived from late embryonic stages of Drosophila melanogaster.. Journal of embryology and experimental morphology. 1972-04, 27 (2): 353–65 [2019-12-26]. PMID 4625067. （原始内容存档于2019-12-26）.
^ Pamboukian, MM; Jorge, SA; Santos, MG; Yokomizo, AY; Pereira, CA; Tonso, A. Insect cells respiratory activity in bioreactor.. Cytotechnology. 2008-05, 57 (1): 37–44 [2019-12-26]. PMID 19003170. doi:10.1007/s10616-007-9118-8. （原始内容存档于2019-12-26）.
^ Moraes, AM; Jorge, SA; Astray, RM; Suazo, CA; Calderón Riquelme, CE; Augusto, EF; Tonso, A; Pamboukian, MM; Piccoli, RA; Barral, MF; Pereira, CA. Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development.. Biotechnology advances. NaN, 30 (3): 613–28 [2019-12-26]. PMID 22079894. doi:10.1016/j.biotechadv.2011.10.009. （原始内容存档于2019-12-26）. 请检查|date=中的日期值 (帮助)
^ Fasbender, F; Claus, M; Wingert, S; Sandusky, M; Watzl, C. Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes.. Frontiers in immunology. 2017, 8: 789 [2019-12-26]. PMID 28736554. doi:10.3389/fimmu.2017.00789. （原始内容存档于2019-12-26）.
^ Yang, J; Reth, M. Drosophila S2 Schneider cells: a useful tool for rebuilding and redesigning approaches in synthetic biology.. Methods in molecular biology (Clifton, N.J.). 2012, 813: 331–41 [2019-12-26]. PMID 22083752. doi:10.1007/978-1-61779-412-4_20. （原始内容存档于2019-12-26）.

外部链接

Cellosaurus entry for Schneider 2（页面存档备份，存于互联网档案馆）

[1] Schneider, I. Cell lines derived from late embryonic stages of Drosophila melanogaster.. Journal of embryology and experimental morphology. 1972-04, 27 (2): 353–65 [2019-12-26]. PMID 4625067. （原始内容存档于2019-12-26）.

[2] Pamboukian, MM; Jorge, SA; Santos, MG; Yokomizo, AY; Pereira, CA; Tonso, A. Insect cells respiratory activity in bioreactor.. Cytotechnology. 2008-05, 57 (1): 37–44 [2019-12-26]. PMID 19003170. doi:10.1007/s10616-007-9118-8. （原始内容存档于2019-12-26）.

[3] Moraes, AM; Jorge, SA; Astray, RM; Suazo, CA; Calderón Riquelme, CE; Augusto, EF; Tonso, A; Pamboukian, MM; Piccoli, RA; Barral, MF; Pereira, CA. Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development.. Biotechnology advances. NaN, 30 (3): 613–28 [2019-12-26]. PMID 22079894. doi:10.1016/j.biotechadv.2011.10.009. （原始内容存档于2019-12-26）. 请检查|date=中的日期值 (帮助)

[4] Fasbender, F; Claus, M; Wingert, S; Sandusky, M; Watzl, C. Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes.. Frontiers in immunology. 2017, 8: 789 [2019-12-26]. PMID 28736554. doi:10.3389/fimmu.2017.00789. （原始内容存档于2019-12-26）.

[5] Yang, J; Reth, M. Drosophila S2 Schneider cells: a useful tool for rebuilding and redesigning approaches in synthetic biology.. Methods in molecular biology (Clifton, N.J.). 2012, 813: 331–41 [2019-12-26]. PMID 22083752. doi:10.1007/978-1-61779-412-4_20. （原始内容存档于2019-12-26）.

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