CHO-K1

CHO-K1（CHO cell clone K1）是源自成年中國倉鼠卵巢的二倍體上皮樣細胞系，並且是CHO細胞（源自中國倉鼠卵巢深入活體組織切片）衍生的野生型亞克隆。目前已知利用甲磺酸乙酯誘變CHO-K1細胞後，會產生缺乏二氫葉酸還原酶（DHFR）活性的CHO-DXB11細胞^[1] 。此外，源自CHO-K1細胞的細胞系的基因組可能曾有着大規模的染色體重新排列，甚至已知它的克隆群體也可以分化為異質亞族群（heterogeneous subpopulations）^[2]^[3]。

據報導，CHO-K1細胞的染色體計數各有不同^[4]，可能是因為它的基因組不穩定。有研究就在CHO-K1細胞中發現了21條染色體^[5]，並且在在CHO-K1細胞的基因組中，鑑定出與大多數人類硫酸乙酰肝素葡糖胺O-磺基轉移酶（human heparan sulfate glucosamine O-sulfotransferases）的同源物^[5]，鑑定結果與以前的研究^[6]^[7]一致。

參考資料

^ Urlaub, G; Chasin, LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.. Proceedings of the National Academy of Sciences of the United States of America. 1980-07, 77 (7): 4216–20 [2020-01-09]. PMID 6933469. doi:10.1073/pnas.77.7.4216. （原始內容存檔於2020-02-23）.
^ M. Derouazi; D. Martinet; N. Besuchet; R. Flaction; M. Wicht; M. Bertschinger; D. Hacker; J. Beckmann; F.M. Wurm. Stability and Cytogenetic Characterization of Recombinant CHO Cell Lines Established by Microinjection and Calcium Phosphate Transfection. Cell Technology for Cell Products. 2007: 443–446. doi:10.1007/978-1-4020-5476-1_73.
^ Pilbrough, W; Munro, TP; Gray, P. Intraclonal protein expression heterogeneity in recombinant CHO cells.. PloS one. 2009-12-23, 4 (12): e8432 [2020-02-23]. PMID 20037651. doi:10.1371/journal.pone.0008432. （原始內容存檔於2020-02-23）.
^ Deaven, LL; Petersen, DF. The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses.. Chromosoma. 1973, 41 (2): 129–44 [2020-02-23]. PMID 4120885. doi:10.1007/bf00319690. （原始內容存檔於2020-02-23）.
^ ^5.0 ^5.1 Xu, X; Nagarajan, H; Lewis, NE; Pan, S; Cai, Z; Liu, X; Chen, W; Xie, M; Wang, W; Hammond, S; Andersen, MR; Neff, N; Passarelli, B; Koh, W; Fan, HC; Wang, J; Gui, Y; Lee, KH; Betenbaugh, MJ; Quake, SR; Famili, I; Palsson, BO; Wang, J. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.. Nature biotechnology. 2011-07-31, 29 (8): 735–41 [2020-02-23]. PMID 21804562. doi:10.1038/nbt.1932. （原始內容存檔於2020-02-23）.
^ Kobayashi, M; Habuchi, H; Yoneda, M; Habuchi, O; Kimata, K. Molecular cloning and expression of Chinese hamster ovary cell heparan-sulfate 2-sulfotransferase.. The Journal of biological chemistry. 1997-05-23, 272 (21): 13980–5 [2020-02-23]. PMID 9153262. doi:10.1074/jbc.272.21.13980. （原始內容存檔於2020-02-23）.
^ Habuchi, H; Kobayashi, M; Kimata, K. Molecular characterization and expression of heparan-sulfate 6-sulfotransferase. Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells.. The Journal of biological chemistry. 1998-04-10, 273 (15): 9208–13 [2020-02-23]. PMID 9535912. doi:10.1074/jbc.273.15.9208. （原始內容存檔於2020-02-23）.

外部連結

Cellosaurus entry for CHO-K1（頁面存檔備份，存於網際網路檔案館）

[1] Urlaub, G; Chasin, LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.. Proceedings of the National Academy of Sciences of the United States of America. 1980-07, 77 (7): 4216–20 [2020-01-09]. PMID 6933469. doi:10.1073/pnas.77.7.4216. （原始內容存檔於2020-02-23）.

[2] M. Derouazi; D. Martinet; N. Besuchet; R. Flaction; M. Wicht; M. Bertschinger; D. Hacker; J. Beckmann; F.M. Wurm. Stability and Cytogenetic Characterization of Recombinant CHO Cell Lines Established by Microinjection and Calcium Phosphate Transfection. Cell Technology for Cell Products. 2007: 443–446. doi:10.1007/978-1-4020-5476-1_73.

[3] Pilbrough, W; Munro, TP; Gray, P. Intraclonal protein expression heterogeneity in recombinant CHO cells.. PloS one. 2009-12-23, 4 (12): e8432 [2020-02-23]. PMID 20037651. doi:10.1371/journal.pone.0008432. （原始內容存檔於2020-02-23）.

[4] Deaven, LL; Petersen, DF. The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses.. Chromosoma. 1973, 41 (2): 129–44 [2020-02-23]. PMID 4120885. doi:10.1007/bf00319690. （原始內容存檔於2020-02-23）.

[Xu-5] 5.0 ^5.1 Xu, X; Nagarajan, H; Lewis, NE; Pan, S; Cai, Z; Liu, X; Chen, W; Xie, M; Wang, W; Hammond, S; Andersen, MR; Neff, N; Passarelli, B; Koh, W; Fan, HC; Wang, J; Gui, Y; Lee, KH; Betenbaugh, MJ; Quake, SR; Famili, I; Palsson, BO; Wang, J. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.. Nature biotechnology. 2011-07-31, 29 (8): 735–41 [2020-02-23]. PMID 21804562. doi:10.1038/nbt.1932. （原始內容存檔於2020-02-23）.

[6] Kobayashi, M; Habuchi, H; Yoneda, M; Habuchi, O; Kimata, K. Molecular cloning and expression of Chinese hamster ovary cell heparan-sulfate 2-sulfotransferase.. The Journal of biological chemistry. 1997-05-23, 272 (21): 13980–5 [2020-02-23]. PMID 9153262. doi:10.1074/jbc.272.21.13980. （原始內容存檔於2020-02-23）.

[7] Habuchi, H; Kobayashi, M; Kimata, K. Molecular characterization and expression of heparan-sulfate 6-sulfotransferase. Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells.. The Journal of biological chemistry. 1998-04-10, 273 (15): 9208–13 [2020-02-23]. PMID 9535912. doi:10.1074/jbc.273.15.9208. （原始內容存檔於2020-02-23）.

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