体细胞核移植

体细胞核移植（Somatic cell nuclear transfer，简称：SCNT），又称体细胞克隆，是遗传学及发育生物学实验之中一种使用体细胞和卵细胞培育胚胎的常用技术手段。该技术提取去核卵母细胞，并把供体体细胞核移入卵母细胞中。体细胞核移植技术被应用于临床治疗及克隆技术中。世界上第一只克隆哺乳动物多利羊即是依靠体细胞核移植技术诞生。2018年1月，中国科学院神经科学研究所非人灵长类平台的研究团队宣布成功通过体细胞核移植技术克隆出两只食蟹猴中中、华华。^[1]

介绍

细胞核移植技术，就是将供体细胞核移入除去核的卵母细胞中，使后者不经过精子穿透等有性过程即无性繁殖即可被激活、分裂并发育成新个体，使得核供体的基因得到完全复制。^[2]1996年世界上第一个使用该技术克隆的动物多利羊诞生。^[3]

参考文献

^ Liu, Zhen; et al. Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer. Cell (journal). 24 January 2018 [24 January 2018]. doi:10.1016/j.cell.2018.01.020. （原始内容存档于2018-02-08）.
^ Somatic cell nuclear transfer | biology and technology. Encyclopedia Britannica. [27 January 2018]. （原始内容存档于2018-01-28）（英语）.
^ Somatic Cell Nuclear Transfer - Embryology. embryology.med.unsw.edu.au. [27 January 2018]. （原始内容存档于2018-01-28）.

延伸阅读

Wilmut I, Beaujean N, de Sousa PA, et al. Somatic cell nuclear transfer. Nature. October 2002, 419 (6907): 583–6. PMID 12374931. doi:10.1038/nature01079.
Kikyo N, Wolffe AP. Reprogramming nuclei: insights from cloning, nuclear transfer and heterokaryons. J. Cell Sci. 113. January 2000,. ( Pt 1): 11–20. PMID 10591621.
Tian XC, Kubota C, Enright B, Yang X. Cloning animals by somatic cell nuclear transfer—biological factors. Reprod. Biol. Endocrinol. November 2003, 1 (1): 98. PMC 521203  . PMID 14614770. doi:10.1186/1477-7827-1-98.
Gurdon JB, Byrne JA, Simonsson S. Nuclear reprogramming and stem cell creation. Proc. Natl. Acad. Sci. U.S.A. 100. September 2003,. Suppl 1 (90001): 11819–22. PMC 304092  . PMID 12920185. doi:10.1073/pnas.1834207100.

参见

外部链接

Research Cloning: Medical and scientific, legal and ethical aspects^{[失效链接]}
The Basics: Stem Cells and Public Policy The Century Foundation, June 2005
"Research Cloning Basic Science", Center for Genetics and Society, (Last modified October 4, 2004, retrieved October 6, 2006)
Cloning: present uses and promises National Institutes of Health, Paper giving background information on cloning in general and SCNT from The Office of Science Policy Analysis.
Nuclear Transfer – Stem Cells or Somatic Cell Nuclear Transfer (SCNT) The International Society for Stem Cell Research
The Hinxton Group: An International Consortium on Stem Cells, Ethics & Law（页面存档备份，存于互联网档案馆）

[CELL-20180124-1] Liu, Zhen; et al. Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer. Cell (journal). 24 January 2018 [24 January 2018]. doi:10.1016/j.cell.2018.01.020. （原始内容存档于2018-02-08）.

[2] Somatic cell nuclear transfer | biology and technology. Encyclopedia Britannica. [27 January 2018]. （原始内容存档于2018-01-28）（英语）.

[3] Somatic Cell Nuclear Transfer - Embryology. embryology.med.unsw.edu.au. [27 January 2018]. （原始内容存档于2018-01-28）.

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