EcoRI
EcoRI(“I”是罗马数字“1”)是一种核酸酶,来自某些特定品系的大肠杆菌(E. coli,也是其名称由来),是此种细菌体内参与限制修饰系统的酵素。
![](http://upload.wikimedia.org/wikipedia/commons/thumb/7/78/EcoRI_restriction_enzyme_recognition_site.svg/220px-EcoRI_restriction_enzyme_recognition_site.svg.png)
![](http://upload.wikimedia.org/wikipedia/commons/thumb/7/71/ECOR1_Crystal_Structure.rsh.png/220px-ECOR1_Crystal_Structure.rsh.png)
在分子生物学或其他分子层次的生物学研究上,EcoRI是一种常用的限制酶,可将特定序列的DNA切割成片段,切割后的小片段端点为5'端突出的黏状末端。
参见
编辑参考文献
编辑- Bitinaite, J., D. A. Wah, Aggarwal, A. K., Schildkraut, I. (1998). "FokI dimerization is required for DNA cleavage." Proc Natl Acad Sci U S A 95(18): 10570-5.
- Pingoud, A., Jeltsch, A. (2001) Structure and function of type II restriction endonucleases, Nucl. Acids Res. 29. 18, 3705-3727.
- McClarin, J.A., Frederick, C.A., Wang, B.C., Greene, P., Boyer, H.W., Grable, J., Rosenberg, J.M. (1986) Structure of the DNA-EcoRI endonucleases recognition complex at 3 Å resolution, Science. 234. 4783, 1526-1542.
- Kurpiewski, M.R., Engler, L.E., Wozniak, L.A., Kobylanska, A., Koziolkiewicz, M., Stec, W.J, Jen-Jacobson, L. (2004) Mechanisms of coupling between DNA recognition and catalysis in EcoRI endonucleases, Structure 12. 1775-1788.