J558L细胞(J558L cells)是从BALB/c小鼠中提取的B细胞淋巴瘤细胞系,最初由剑桥大学的M. Bruggeman 和 M.S. Neuberger分离[1][2]。它是一种稳定的B细胞系,可用来持续表达抗体[3],可在添加双抗与10%胎牛血清DMEM培养基中悬浮培养。

J558L细胞(小鼠B淋巴瘤细胞)

参考资料

编辑
  1. ^ http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=88032902&collection=ecacc_gc
  2. ^ Satoru Harumiya, Aya Yoshino, Koji Hayashizaki, Kazuya Mizuno, Hidetaka Yakura, Takahiro Adachi. A system for reconstructing B cell antigen receptor signaling in the mouse myeloma J558L cell line. Archives of Biochemistry and Biophysics. 2013-05, 533 (1-2): 18–24 [2020-01-28]. doi:10.1016/j.abb.2013.02.008. (原始内容存档于2018-06-27) (英语). 
  3. ^ V. T. Oi, S. L. Morrison, L. A. Herzenberg, P. Berg. Immunoglobulin gene expression in transformed lymphoid cells.. Proceedings of the National Academy of Sciences. 1983-02-01, 80 (3): 825–829 [2020-01-28]. ISSN 0027-8424. PMC 393473 . PMID 6402777. doi:10.1073/pnas.80.3.825 (英语). 

外部链接

编辑