MB49

MB49是源自成年C57BL/6 小鼠的膀胱移行細胞癌（英語：Transitional cell carcinoma）細胞系^[1]，建立細胞系的過程使用了DMBA進行誘導。儘管供體小鼠是雄性，但是進行核型分析後發現MB49細胞丟失Y染色體，因此不會表達雄性特異性抗原。最近的一項研究就評估MB49細胞對睪酮及人絨毛膜促性腺激素的敏感性，發現雄性小鼠MB49腫瘤的生長明顯大於雌性小鼠，並且發現懷孕不會影響雌性小鼠MB49腫瘤生長的大小或速度^[2]。MB49細胞無法在體外響應人絨毛膜促性腺激素進行增殖，並且缺乏促性腺激素功能受體^[2]。除此之外，二氫睪酮在體外會強烈刺激MB49細胞的生長^[2]。

研究用途

MB49細胞是現時唯一被使用的鼠膀胱癌細胞系^[3]^[4]，因為另一個以前常用的MBT-2細胞，後來被發現已受複製的C型逆轉錄病毒的污染，而使該細胞系成為不可靠的免疫測定模型^[5]^[6]。缺失Y染色體和缺乏雄性特異性抗原的表達是膀胱癌內的常見早期事件，因此符合有關條件的MB49細胞是一個合適而有效的模型系統，目前已知其可以用作膀胱癌的體外和體內模型^[7]，並且因具有高轉移潛力而可用於進一步的膀胱癌遷移研究。MB49細胞幾乎沒有或完全沒有MHC1類分子和MHC2類分子，但是使用干擾素刺激它時，就會使其產生MHC II類抗原^[8]。

參考資料

^ Summerhayes, IC; Franks, LM. Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro.. Journal of the National Cancer Institute. 1979-04, 62 (4): 1017–23 [2020-02-15]. PMID 107359. （原始內容存檔於2020-02-15）.
^ ^2.0 ^2.1 ^2.2 White-Gilbertson, S; Davis, M; Voelkel-Johnson, C; Kasman, LM. Sex differences in the MB49 syngeneic, murine model of bladder cancer.. Bladder. 2016, 3 (1) [2020-02-15]. PMID 26998503. doi:10.14440/bladder.2016.73. （原始內容存檔於2020-02-15）.
^ Smolensky, D; Rathore, K; Cekanova, M. Molecular targets in urothelial cancer: detection, treatment, and animal models of bladder cancer.. Drug design, development and therapy. 2016, 10: 3305–3322 [2020-02-15]. PMID 27784990. doi:10.2147/DDDT.S112113. （原始內容存檔於2020-02-15）.
^ Zhang, N; Li, D; Shao, J; Wang, X. Animal models for bladder cancer: The model establishment and evaluation (Review).. Oncology letters. 2015-04, 9 (4): 1515–1519 [2020-02-15]. PMID 25788992. doi:10.3892/ol.2015.2888. （原始內容存檔於2020-02-15）.
^ De Boer, EC; Teppema, JS; Steerenberg, PA; De Jong, WH. Retrovirus type C in the mouse bladder carcinoma cell line MBT-2.. The Journal of urology. 2000-06, 163 (6): 1999–2001 [2020-02-15]. PMID 10799246. doi:10.1097/00005392-200006000-00109. （原始內容存檔於2020-02-15）.
^ Kasman, L; Voelkel-Johnson, C. An orthotopic bladder cancer model for gene delivery studies.. Journal of visualized experiments : JoVE. 2013-12-01, (82): 50181 [2020-02-15]. PMID 24326612. doi:10.3791/50181. （原始內容存檔於2020-02-15）.
^ Chen, F; Zhang, G; Cao, Y; Hessner, MJ; See, WA. MB49 murine urothelial carcinoma: molecular and phenotypic comparison to human cell lines as a model of the direct tumor response to bacillus Calmette-Guerin.. The Journal of urology. 2009-12, 182 (6): 2932–7 [2020-02-15]. PMID 19853870. doi:10.1016/j.juro.2009.08.018.
^ MB49 Cell Line. Ximbio. [2020-02-15]. （原始內容存檔於2020-02-15）（英語）.

外部連結

Cellosaurus entry for MB49 （頁面存檔備份，存於網際網路檔案館）

[1] Summerhayes, IC; Franks, LM. Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro.. Journal of the National Cancer Institute. 1979-04, 62 (4): 1017–23 [2020-02-15]. PMID 107359. （原始內容存檔於2020-02-15）.

[White-2] 2.0 ^2.1 ^2.2 White-Gilbertson, S; Davis, M; Voelkel-Johnson, C; Kasman, LM. Sex differences in the MB49 syngeneic, murine model of bladder cancer.. Bladder. 2016, 3 (1) [2020-02-15]. PMID 26998503. doi:10.14440/bladder.2016.73. （原始內容存檔於2020-02-15）.

[3] Smolensky, D; Rathore, K; Cekanova, M. Molecular targets in urothelial cancer: detection, treatment, and animal models of bladder cancer.. Drug design, development and therapy. 2016, 10: 3305–3322 [2020-02-15]. PMID 27784990. doi:10.2147/DDDT.S112113. （原始內容存檔於2020-02-15）.

[4] Zhang, N; Li, D; Shao, J; Wang, X. Animal models for bladder cancer: The model establishment and evaluation (Review).. Oncology letters. 2015-04, 9 (4): 1515–1519 [2020-02-15]. PMID 25788992. doi:10.3892/ol.2015.2888. （原始內容存檔於2020-02-15）.

[5] De Boer, EC; Teppema, JS; Steerenberg, PA; De Jong, WH. Retrovirus type C in the mouse bladder carcinoma cell line MBT-2.. The Journal of urology. 2000-06, 163 (6): 1999–2001 [2020-02-15]. PMID 10799246. doi:10.1097/00005392-200006000-00109. （原始內容存檔於2020-02-15）.

[6] Kasman, L; Voelkel-Johnson, C. An orthotopic bladder cancer model for gene delivery studies.. Journal of visualized experiments : JoVE. 2013-12-01, (82): 50181 [2020-02-15]. PMID 24326612. doi:10.3791/50181. （原始內容存檔於2020-02-15）.

[7] Chen, F; Zhang, G; Cao, Y; Hessner, MJ; See, WA. MB49 murine urothelial carcinoma: molecular and phenotypic comparison to human cell lines as a model of the direct tumor response to bacillus Calmette-Guerin.. The Journal of urology. 2009-12, 182 (6): 2932–7 [2020-02-15]. PMID 19853870. doi:10.1016/j.juro.2009.08.018.

[8] MB49 Cell Line. Ximbio. [2020-02-15]. （原始內容存檔於2020-02-15）（英語）.

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