HindIII
HindIII是一种发现于细菌流感嗜血杆菌(Haemophilus influenzae,故缩写为Hin)的II型限制酶,其识别序列为[1]
HindIII restriction endonuclease | |||||||||
---|---|---|---|---|---|---|---|---|---|
![]() | |||||||||
鉴定 | |||||||||
标志 | RE_Hindiii | ||||||||
Pfam | PF09518(旧版) | ||||||||
InterPro | IPR019043 | ||||||||
|
hindIIIR type II restriction endonuclease | |
---|---|
标识 | |
生物 | |
符号 | hindIIIR |
Entrez | 950303 |
PDB | 2e52 More structures |
UniProt | P43870 |
其他数据 | |
EC number | 3.1.21.4 |
![](http://upload.wikimedia.org/wikipedia/commons/thumb/e/e0/HindIII_Restriction_site_and_sticky_ends_vector.svg/220px-HindIII_Restriction_site_and_sticky_ends_vector.svg.png)
5'AAGCTT 3'TTCGAA
切割产生的粘性末端为
5'---A AGCTT---3' 3'---TTCGA A---5'
参考文献
编辑- ^ Tang, D; et al. Mutational analyses of restriction endonuclease-HindIII mutant E86K with higher activity and altered specificity. Protein Engineering. 2000, 13 (4): 283–9 [2020-09-21]. PMID 10810160. doi:10.1093/protein/13.4.283 . (原始内容存档于2020-03-13).