磷酸甘油酸脱氢酶
位於1號人類染色體的基因
磷酸甘油酸脱氢酶（英语：phosphoglycerate dehydrogenase，EC 1.1.1.95 （页面存档备份，存于互联网档案馆）），是一种以NAD+或NADP+为受体、作用于供体CH-OH基团上的氧化还原酶。这种酶能催化以下两种酶促反应：
| 磷酸甘油酸脱氢酶 | |||||||||
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| 识别码 | |||||||||
| EC编号 | 1.1.1.95 | ||||||||
| CAS号 | 9075-29-0 | ||||||||
| 数据库 | |||||||||
| IntEnz | IntEnz浏览 | ||||||||
| BRENDA | BRENDA入口 | ||||||||
| ExPASy | NiceZyme浏览 | ||||||||
| KEGG | KEGG入口 | ||||||||
| MetaCyc | 代谢路径 | ||||||||
| PRIAM | 概述 | ||||||||
| PDB | RCSB PDB PDBj PDBe PDBsum | ||||||||
| 基因本体 | AmiGO / EGO | ||||||||
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- 3-磷酸化-D-甘油酸 + NAD+ 3-磷酸羟基丙酮酸 + NADH + H+
- 2-羟基戊二酸 + NAD+ α-酮戊二酸 + NADH + H+
在大肠杆菌（Escherichia coli）中，磷酸甘油酸脱氢酶参与催化丝氨酸生物合成的磷酸丝氨酸途径中的第一步，[1]上述可逆反应主要逆向进行。磷酸甘油酸脱氢酶可被丝氨酸或甘氨酸抑制。这种酶很不寻常，因为它还能像D-2-羟基戊二酸脱氢酶和L-2-羟基戊二酸脱氢酶一样还原α-酮戊二酸，其中，D型底物更适合磷酸甘油酸脱氢酶。[2]
参考文献
编辑- ^ Pizer, L.I. The mechanism of end product inhibition of serine biosynthesis. I. Purification and kinetics of phosphoglycerate dehydrogenase. J. Biol. Chem. 1986, 243: 2081–2089. PMID 4384871.
- ^ Zhao, G. and Winkler, M.E. A novel α-ketoglutarate reductase activity of the serA-encoded 3-phosphoglycerate dehydrogenase of Escherichia coli K-12 and its possible implications for human 2-hydroxyglutaric aciduria. J. Bacteriol. 1996, 178: 232–239. PMID 8550422.
- Sugimoto, E. and Pizer, L.I. The mechanism of end product inhibition of serine biosynthesis. I. Purification and kinetics of phosphoglycerate dehydrogenase. J. Biol. Chem. 1968, 243: 2081–1089. PMID 4384871.
- Schuller, D.J., Grant, G.A. and Banaszak, L.J. The allosteric ligand site in the Vmax-type cooperative enzyme phosphoglycerate dehydrogenase. Nat. Struct. Biol. 1995, 2: 69–76. PMID 7719856.
扩展阅读
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- Jaeken J, Detheux M, Van Maldergem L; et al. 3-Phosphoglycerate dehydrogenase deficiency: an inborn error of serine biosynthesis.. Arch. Dis. Child. 1996, 74 (6): 542–545. PMC 1511571 . PMID 8758134. doi:10.1136/adc.74.6.542.
